Is the oxygen level monitored in the incubator important?

In our lab. we have 2 versions of cell culture incubator, one is an old fashion without oxygen controlled, the another is new designed with tri gases; nitrogen, oxygen and CO2. Will the new...

21 May 2015 9,550 3 View

Is the oxygen level monitored in the incubator important?

In our lab. we have 2 versions of cell culture incubator, one is an old fashion without oxygen controlled, the another is new designed with tri gases; nitrogen, oxygen and CO2. Will the new...

04 May 2015 4,005 4 View

Where might one buy particulate matter (PM) 2.5 um?

10 November 2014 2,140 10 View

Where might one buy particulate matter (PM) 2.5 um?

03 November 2014 3,893 9 View

How much and what kind of Matrigel is needed to coat a plate?

I plan to grow isolated circulating tumor cells (CTCs) on a Matrigel-coated well plate. I have learned that there are 2 types of Matrigel-coated well plates and that one is thick and the other...

03 April 2014 7,565 6 View

Health impact of autogas, LPG fueled car?

Currently, here in Thailand we are using a lot of cars fueled with LPG as an alternative source of energy. Since its price is cheaper than gasohol and diesel, increasingly, people turn their cars...

10 November 2013 563 2 View

Does anyone have experience using a 3D cell culture novel screening method for anticancer agents?

3D cell culture in liquid form. This is quite new to me. Usually, I always heard about 3D collagen gel model for cell profiration or migration. In this new case they (Nissan Chemical Industries)...

24 July 2013 992 3 View

Does anyone have experience using a 3D cell culture novel screening method for anticancer agents?

3D cell culture in liquid form. This is quite new to me. Usually, I always heard about 3D collagen gel model for cell profiration or migration. In this new case they (Nissan Chemical Industries)...

06 July 2013 5,892 3 View

What is a good performance for Cytocentrifuge?

We are looking for a brand new cytocentrifuge which will be used for cell sedimentation and fixation. Since this is very new to me, are there any comments or criteria should I have to...

24 April 2013 4,176 3 View

Any comments on circulating tumor cell isolation by size, a commercial product.

I have learnt from a website who make a cell isolation product named ScreenCell that it can be used to isolate tumor cells circulating in blood current by filtation. It seems to me that this...

01 February 2013 5,851 28 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

No title

02 March 2021 3,060 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View