Pelzek et al. (2008) entitled Isolation of Bacteriophages from Environmental Sources, and Creation and Functional Screening of Phage DNA Libraries describes protocols for the isolation of phages from environmental samples however, it requires numerous materials and follows many steps. I am requesting for an alternative protocol which optimizes them.
Dear Katherine,
I think that a new paper at recent time, than you should consult from that article. I think important thing is to find out indicator bacteria, then other steps is not so difficult, just only practice patiently and then success.
There are differences in protocol, depending on the source material (e.g., soil, or water). There is an excellent protocol for dsDNA phage isolation from soil in Williamson et al (2003), which avoids the need for the rather tedious 'traditional' protocol (suspension in buffer, agitation, settling, sequential filtration, concentration by Tangential Flow filtration, PVPP precipitation or centrifugation to recover VLPs).
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