As much as trios or as much as cases?
Is it for a functional gain or a nonsense anatomical variation
05 June 2014 7,565 11 View
Which explain different types of epigenetic changes and their relation with gene function and disease states.
05 June 2014 5,883 4 View
Is there any paper or website which explain the usage of NGS data softwares step by step for rookies?
03 April 2014 3,438 2 View
I would like to know if it is possible to do whole-exome sequencing from paraffin-embedded tissues, and, if so, what the protocol is.
11 December 2013 5,549 4 View
I am planning exome sequencing for a disease which to find a family is extremely difficult. That is why we have to study on sporadic cases. At this point which approach is better. As we think...
11 December 2013 8,261 2 View
.
11 December 2013 7,492 8 View
Can you suggest any software?
11 December 2013 2,056 2 View
I am interested in suggestions for tools for whole-exome sequencing.
11 December 2013 5,394 4 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...
24 February 2021 8,856 3 View
I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...
24 February 2021 4,397 3 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View
Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?
23 February 2021 3,840 3 View
Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks
23 February 2021 6,518 3 View
I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...
19 February 2021 7,006 5 View
