As much as trios or as much as cases?
Is it for a functional gain or a nonsense anatomical variation
05 June 2014 7,612 11 View
Which explain different types of epigenetic changes and their relation with gene function and disease states.
05 June 2014 5,936 4 View
Is there any paper or website which explain the usage of NGS data softwares step by step for rookies?
03 April 2014 3,474 2 View
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11 December 2013 7,544 8 View
I would like to know if it is possible to do whole-exome sequencing from paraffin-embedded tissues, and, if so, what the protocol is.
11 December 2013 5,601 4 View
I am planning exome sequencing for a disease which to find a family is extremely difficult. That is why we have to study on sporadic cases. At this point which approach is better. As we think...
11 December 2013 8,306 2 View
Can you suggest any software?
11 December 2013 2,109 2 View
I am interested in suggestions for tools for whole-exome sequencing.
11 December 2013 5,440 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
24 July 2024 9,337 3 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...
20 July 2024 5,405 2 View
Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...
18 July 2024 1,579 2 View
How is biomass energy essential in the power generation in a country.
08 July 2024 9,477 2 View
