What immunoprecipitation elution buffer is compatible with ELISA?

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Steingrimur Stefansson

Hi Masaya Oshima . You could try low pH buffers e.g. 10 mM acetic acid to elute and then neutralize for the ELISA.

Junjie Shao

For the ELISA for IP sample, the concern is the antibody you used for IP. the detection antibody should be not the same (not from the same spec), the conjugate 2nd antibody maybe recognizes the IP antibody.