Is it possible to freeze secondary antibodies repeatedly?

18 June 2021 1 6K Report

Is it possible to freeze secondary antibodies repeatedly? And then how long can we use it? We use lgG StarBright Brue Fluorescent Secondary Antibodies.

Steingrimur Stefansson

Masaya Hirota . It is not recommended to do multiple freeze-thaws of antibodies. They denature and form aggregates that you need to spin out before using.

Either store them at 4oC with azide or at -20oC in 20% glycerol.

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