What immune response would occur when skin cells of mice is taken out, transfecetd (E.coli derived gene) and injected back to same mice ?

06 May 2021 0 414 Report

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Science method

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Astrology and psychology ?any connection?

Psychology too guarantees holistic healing and success in healthy life style.Astrology too mentions the same with remedies too .

26 December 2023 9,336 3 View

Statistical method for Macro-invertebrates species presence-absence data?

Hey Happy New Year Greetings! I have species presence-absence data of macro-invertebrates of an Himalayan stream from 7 different sites and two season. What is the best way to represent this...

06 January 2023 4,621 3 View

How to quantify spheroidenone - HPLC analysis?

Can anyone suggest the HPLC method to quantify spheroidenone carotenoid with a DAD detector and an Agilent zorbax SB-C18 column?

30 May 2022 6,188 5 View

What factor affecting the mobility of DNA protein complex in agrose gel?

what factor affects the mobility of DNA protein complex in agarose gel?

18 April 2022 383 4 View

Anyone suggest electrophoresis for interaction of Protein with DNA give detail procedure and material?????

01 April 2022 3,283 1 View

How can i perform RDA analysis from two different set of data using PAST software?

I have to correlate the antibiotic resistance present in my samples with the bacterial diversity. I have CFU data for antibiotic resistance and amplicon sequencing data for bacterial diversity for...

10 July 2021 9,716 3 View

How to achieve resistance on Cultural Change in Organization Change Management?

Controls on Cultural change in Organizational Change Management and how to add good questions for survey

16 May 2021 7,838 8 View

Is Research Square Preprint Plateform reliable?

This plateform provides tracking, editing, etc., services for our manuscript. If anyone knows about it then please inform me. Thanks.

09 May 2021 2,848 0 View

How to perform Spectral Matching in Frequency Domain ?

I tried to match the earthquake response spectra to design spectra using spectral matching in frequency domain . After calculating the modification factor( the ratio of spectral acceleration at...

06 March 2021 6,407 0 View

Can we calculate percent N or N content by ammonia (mg/l)values in cattle dung?

01 February 2021 2,966 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View