Hello every one looking for recommendations on dissertation?

Hello every one, This is deepak studying masters in human resource management and employee relations... I do have some ideas for my thesis but still I'm looking for some interesting...

28 November 2018 9,325 3 View

Which is the best modulation technique for wsn ?

modulation schemes for wsn to reduce power

08 March 2016 618 3 View

What is the protection window for an inactivated viral vaccine normally?

Vaccinations of 4 Camelus dromederius starting 3rd Aug followed by 3 boosters at 14 day intervals against the foot and mouth disease virus (146S particle and recombinant nonstructural protein...

08 December 2014 4,385 7 View

How do you measure absorption spectra of single and isolated CdSe quantum dots spread over a 200nm thick silver film?

Diameter of CdSe quantum dot is about 5nm.

26 November 2014 4,125 3 View

Can anyone help with an antibiotic sensitivity test for Pasteurella multocida isolates by disc diffusion method as per CLSI protocol?

I am not able to get the interpretation criteria for disc diffusion method as suggested by Clinical and Laboratory Standards Institute (CLSI) for Pasteurella multocida. Can anyone provide me the...

28 April 2014 369 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Which distance should I measure to calculate the voltage to use for my agarose gel electrophoresis?

Hi, I've been reading recommendations of which voltage to use for agarose gel electrophoresis and they are often given in V/cm, where the cm refers to the "distance between the anode and cathode"...

14 February 2021 6,463 3 View

Why does the band sizes on Agarose gel electrophoresis differ from the peaks at capillary electrophoresis?

14 February 2021 4,773 4 View

Does sds interfere with immunization?

I want to extract a protein from polyacrylamide gel to be used for immunization. I am wondering if I need to run PAGE since sds might interfere with the immunization process. Thank you

10 February 2021 8,568 3 View

Can anyone suggest some leading universities worldwide researching on nanotechnology for postdoc?

My area of research is synthesis of metal nanoparticles and their applications focusing on protein extraction from complex biological mixtures using SDS page gel electrophoresis technique. Please...

09 February 2021 2,941 3 View

PCR primers bind to specific DNA at specific temperatures but not other temperatures?

I ran PCR in order to analyze a P-element excision on a mutant drosophila line. My results were not conclusive, however that is beside the point. I ran a temperature gradient in order to optimize...

25 January 2021 9,164 11 View