Anyone suggest electrophoresis for interaction of Protein with DNA give detail procedure and material?????

02 April 2022 1 3K Report

Hello Laxmi Kashyup

Electrophoretic Mobility Shift Assay (EMSA) is used to analyze nucleic acid–protein interactions. EMSA is based on the principle that DNA–protein complexes are larger and move slowly when subjected to nondenaturing polyacrylamide gel electrophoresis (PAGE), compared to the unbound (free) DNA probe. Since the rate of DNA migration is shifted or retarded when bound to protein, the assay is also referred to as a gel shift or gel retardation assay.

Please refer to a representative protocol provided in the article below. Commonly used variants and expected outcomes are also discussed in the paper.

Article Hellman, L.M. & Fried, M. Electrophoretic mobility shift ass...

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