The samples will contain nearly 10kDa DNA-part and covalent conjugated 40kDa PEG, we plan to analyze using HPLC. What column should we use for best analysis?
You sample have MW 10kDa DNA- and 40kDa PEG, so you choose column which have more diameter (size), CR4 Column or C4 is best choice and use UV detector for best identification.,
C8 column also possible to use but it slow and get more time difference, so CR4 is best option., do you have any reference result means compare the result after HPLC run, from that you can able to know the time cover.,