Data signal through a twisted pair cable by using different color codes will work ?

will a Data signal through a twisted pair cable by using different color codes will work ?. I am using a shielded twisted pair cable for my Mod bus device. Instead of same color pairs for Data...

02 May 2019 6,361 3 View

How to measure the heat transfer in hollow block?

what instrument is used for measuring heat transfer

06 January 2019 3,087 3 View

How to find the effect of signalling molecule on metabolic pathway?

I am started working on " BMP over breast cancer". I am interested to know, is there any system biology tool available to predict the possibility of BMP interaction over the metabolic pathways (...

28 January 2018 5,271 3 View

Anyone have experience in performing native immunoblot for cell supernatant IP samples?

Can anyone suggest a protocol to verify the oligomerization of secreted protein using immunoprecipitation and native immunoblot.

22 October 2017 2,177 2 View

Is it possible to separate nano particles from polymer?

I synthesized a polymer and when i try to use it polymer for drug delivery the size is more than 200nm. So i did mechanical distribution and separated particles less than 200nm and applied for...

11 February 2015 7,114 6 View

Is there any relationship between crystality & photoluminescence of the material?

My friend told me crystal materials only have luminescence properties. Is it right?

14 January 2015 6,612 4 View

Why are GFPs or RFPs being lost after certain passages?

I have developed 4 stable clones with GFPs and RFPs none of them show up after 20 or 25 passages though they are in selection media? Is it slowly losing the plasmids or is the florescence not...

11 December 2013 1,596 17 View

Is aryl boronic pinacol ester stable to basic conditions?

I have an aryl boronic pinacol ester (Ar-Bpin) which has a phenolic -OH group. I would like to use K2CO3 conditions, as well as NaOH conditions (separate reactions). Question is whether Ar-Bpin...

23 October 2013 8,139 2 View

Click chemistry on multiple azide groups - can anyone help?

I am trying to do a small scale (approximately 20 micromol of azide and 50 micromol of alkyne) click reaction using CuSO4.5H2O (5-10 mol%) and (+) sodium -L-ascorbate (20 mol%). My alkyne and the...

17 July 2013 2,480 31 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View