What happens when 10x sodium borate buffer is used instead of 1x while purifying a protein with HiTrap Protein A?

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Does anyone know why the consumption of extra fat in the diet of gilts can increase the weight only of the ovaries ?

Can the consumption of extra fat (2%) in the diet of prepubertal gilts cause an increase only in the weight of the ovaries? The rest of the uterine structures are not affected (no CL, CA..) Why...

21 January 2021 2,186 3 View

Is a good idea to coat the plates with collagen when working with 3T3-L1 cells??

I'm going to start working with 3T3-L1 cells and I've been reading... many people have problems of attachment of the cells once differentiated and I was wondering if it would be worth it to coat...

27 April 2020 9,513 0 View

Dear Tomás. Are you registering the "transfusion" consume? Do you have register the "massive transfusion protocol" changes? And the tranexamic use?

Dear Tomás I am very interesting in the "blood" management: - massive transfusion protocols - tranexamic use at the trauma scenario (including out hospital) - "haemostasic" packs: the use of...

26 December 2019 5,298 0 View

What is your favorite search engine to find ontologies?

I'm interested in finding ontologies in the domain of sustainable territories

07 January 2015 6,946 13 View

Is plasma transfusion really needed? Is there any evidence about blue methylene inactivated plasma use?

Blue methylene inactivated plasma must be forbidden. Most of the inadequate blood transfusions are plasma ones. Plasma transfusion had more frequent and worse transfusion reactions: it is...

12 November 2012 9,325 14 View

Questionnaire to assess User Experience (UX) and Technology Acceptance in Human-Robot Interaction on industrial contexts

We, at the Design Innovation Centre of Mondragon University, are working to better understand the interaction between humans and robots through a user-focused questionnaire. Our Human-Robot...

01 January 1970 5,750 2 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,898 0 View

How can EDTA be removed from already extracted DNA samples?

Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...

13 July 2024 1,675 3 View