almost certainly not. GC is just one of many factors affecting dna binding so I would expect it to work well if it matches the other criteria such as balanced annealing temperature and no primer diming with the other primer and no self annealing ( hairpin self looping)

Hi,

The GC content in your primers should usually be =/>50%. This is essential for reasonable annealing temperature and efficient primer binding. Therefore, make sure your primers are highly specific to your gene of interest if you have low GC content. Knowing your Tm would help in deducing whether there will me much impact of this on your PCR amplification. If your Tm is good enough, trying amplification for different annealing temperatures should give you a good product.

almost certainly not. GC is just one of many factors affecting dna binding so I would expect it to work well if it matches the other criteria such as balanced annealing temperature and no primer diming with the other primer and no self annealing ( hairpin self looping)

Thank you everyone