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Questions related from Lavanya Ravichandran
am setting up allele specific pcr for cyp21a2 gene that has a pseudogene. i have already amplified the normal gene with specific primers. using this as secondary product i am trying to set up...
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one of the primers i am designing has a gc content only of 34%. will it affect the pcr yield or reaction
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i use primer 3 website. are there other good tools to validate the primers apart from ncbi
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