What extraction buffer is the best for LUMINEX cytokines analyzed in rat's muscle?

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Patricia L. Campos-Ferraz

Thank you, Amir Nawi! I will look it up! I was told that if I make a RIPA buffer without DMSo or other organic solvente, it will be just fine too.

Nikunj Gevariya

RIPA is going to denature your cytokine and if the kit is not made for denatured cytokine then you have be concern. Luminex or Bioplex have the dedicated kit for cell lysis. I would suggest to go with that.