The internet seems divided about whether a 0.5M EDTA solution is irrecoverably compromised if one overshoots the desired pH of 8.0 while adding NaOH. Some say its buffering capacity is permanently reduced if the solution exceeds pH 8.0, while others suggest you can just adjust the pH downward by adding HCl or by increasing the total volume and adding more EDTA.
If the buffering (chelating?) capacity is reduced when the solution exceeds pH 8.0, then why does this not occur locally in the solution while dissolving NaOH pellets or adding NaOH solution to increase the pH in the first place? And what is the actual threshold for ruining the buffering capacity of the EDTA? Is it 8.05? 8.1? 8.2? Is it better to be a hair under 8.0 or a hair over?
(I'm using the 0.5M EDTA to make DESS nematode preservative (Yoder et al., 2006), if that is useful information.)
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