So I am doing DNA extraction from Calendula officinalis and in the step to add the elution buffer in order tu elute your DNA I have always (even though I do plasmid extraction or genomic DNA extraction from plants) elute it in Mili Q watter. But now I have the problema that my extraction do not last, I do my PCR and check if I have DNA but it doesn't; it appers like is degradeted from years.
Now I'm going to elute my DNA in other kind of buffer but I do not know what is the difference between using an elution buffer with Tris-Cl or Tris-HCl or maybe both of them in equals concentrations.
Hope you can help me to solve my doubt!
Thank you in anticipation.
I suggest you to elute your DNA in TE buffer. Tires helps to maintain the pH and EDTA protects it from the degradation caused by Magnesium ions. We use it for the said purpose and the DANA is workable for several months.
Try boiling method for extraction if you are targeting Gram negatives. Use TE buffer instead of the distill water in this method.
Careful with TE, though, depending on what you want to do with your samples down the line. " The purified DNA can also be eluted in TE (10 mM Tris-Cl, 1 mM EDTA, pH 8.0), but the EDTA may inhibit subsequent enzymatic reactions. " (Source: https://www.qiagen.com/ca/resources/faq?id=d8ef773f-6a37-4993-908b-8bbc7bc5c8d6&lang=en )
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