Please what research method will be appropriate for examining impacts of COVID-19 on health outcomes?

I am writing a new proposal for my Thesis. I want to know what of the research methodology will be appropriate.

26 May 2020 1,243 8 View

Securing research funding from industries in developing nations

Greetings, I am soliciting your views on the best way to go in seeking/securing research funding from industries in developing nations. More specifically from low to mid level developing...

04 May 2017 3,595 3 View

Are PCR components and DNA isolation Products from kits stored in -80 C for a long time still viable?

I've been trying to amplify the products for quite a while now and I have difficulty in getting good bands or any bands at all, I'm wondering if it's the storage condition of the components or...

30 August 2016 1,052 7 View

Maintenance of PFGE electrophoresis cell during long breaks (not in use for say 3 months)!?

What is the usual recommended procedure for maintenance of the PFGE electrophoresis cell during long breaks (not in use for 3 months): 1. Clear all the buffer/water from tubings, OR 2. Flush once...

26 April 2016 2,895 7 View

Is anyone using the NEB Lambda ladder PFG Marker?

01 November 2014 5,626 11 View

What are the prospectives of additives for synergism along with antibiotics against antibiotic resistant bacteria?

Synergism along with antibiotics against antibiotic resistant bacteria!

14 September 2014 8,747 8 View

Why am I not able to change/adjust the parameters (in. switch and fin. switch time and total run time) in the power module of CHEF DRIII?

03 September 2014 4,789 3 View

Are buffered solution at neutral pH better than water for resuspending the master stock PRIMERS?

Resuspending the master stock PRIMERS

24 August 2014 3,494 5 View

Among PCR based bacterial typing methods which have you found quite promising?

22 July 2014 9,481 1 View

Covid 19 Pandemic ' impact on digital transformation?

Covid 19 Pandemic and digital transformation

22 February 2021 3,351 5 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Is the logarithmic transformation a necessary step in time series analysis ?

Hello, I'm checking if the log-tranformation of time series data is a necessary step; We all know the aim of such transformation, but in every study (in time series analysis) we found the authors...

15 February 2021 5,655 14 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

How to measure 50uL of cells?

Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...

14 February 2021 6,247 3 View

Mixed ANOVA with some normal and some non-normal data?

I am looking to run a 2x5 Mixed ANOVA to examine Sensory Processing Sensitivity in Highly Sensitive People (HSP) and Non_HSP. My Between-Subjects factor is HSP and Non-HSP. All participants tested...

11 February 2021 1,445 6 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

What is the reference value of THD in BLDC MOTOR speed control ?

i used to control EHO+ANFIS to MRPID to Landsman converter for bldc drive i got 7% but some of them are telling IEEE standards are < 5% is it correct for Drives ?

07 February 2021 5,892 1 View

After transformation of Dh5alpha competent cells I found a cloudy white ppt before plating. Can anyone explain this?

After transformation of Dh5alpha cells by heat shock I grew them at 37 in 200microlitre Terrific Broth (because I had it at hand) and then before plating I found cloudy white ppt in the cell...

03 February 2021 7,867 3 View