The size of the protein is 154KDa his-tagged. I have tried inducing with 0.5mM IPTG for 4hrs at 37 degrees. I have tried different strains BL21-De3 Gene X, shuffle T7, and artic but still no expression. I have also tried 25 degrees overnight. Any suggestions might be helpful.
Wen Liu
This is pretty common, since it is usually not efficient for E. coli to express protein with a size of more than 100 kDa, especially if contains many Cys residues as well.
If you think you have tried all necessary things for E. coli expression system, very likely you need to consider other expression systems such as yeast or insect cells.
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