Amended on 28 May 2017

We have developed "the total serum protein test" by HPLC-SEC-photometric method (please see file; "serum prot deter" using 100 Å pore-diameter Diol-type gel).    Further, we have developed an improved general-protein test using 50 Å pore-diameter Diol-type gel (please see file; HPLC-Surf-SEC protein determination method).   This method is an unique application of short-SEC column which elutes all proteins (Mr larger than c.a. 5000) at V0 (void volume) without any separation.   Similar method has recently been applied onto the fucoidan (polysaccharide of sulphated fucose) determination using 300 Å pore-diameter Diol-type gel(please see file; JCB Fucoidan active transport).  

Usual photometric protein-assay methods and ELISA give sadly false values due to violation of "Lambert-Beer Law".  

Therefore, HPLC method uniquely gives fruitful result; i.e., we have found that leak of UHS-KAP (ultra-high-sulphur keratin-associated proteins) into blood is occurring in the children's alopecia patients via biotin deficiency (low level of biotin (vitamin H) concentration in the blood) (please see file; JMBT Alopecia).

Thus, the HPLC method can be the sole assessor for estimating the causes of many diseases.

Further, I would like to indicate that serum contains much amount of invaded microbes (virus, bacteria, and fungi); i.e., median of healthy serum (n = 3) is 7.4 % (range = 4.52 - 10.8; n = 3), but median of serum of biotin deficiency (n = 5) and common cold (n = 1) patients is 16.7 % (range = 14.3 - 18.5; n = 6), respectively.   This difference in medians is significant (p < 0.05; Mann-Whitney's U test, two-tailed test).  

Serum data has been summarized in a table (file; Table Serum total protein, proteins of invaded microbes, and Alb 1).   These data unexpectedly suggest that total serum protein (TP) is not helpful to assess diseases.   However, precise determination of total serum protein (TP) is indispensable to perform PDMD method; i.e., serum should be precisely diluted to 1.0 mg/mL in order to bind proteins covalently onto the glass filters to apply to automated protein-microsequencer.   Furthermore, this table indicates that biotin therapy does not reduce the amount of invaded microbes at all.   Therefore, biotin therapy should be performed with co-administration of fucoidan in order to reduce these invaded microbes. 

 Tietz, N. (1995). Clinical Guide to laboratory test. Philadelphia: WB Saunders.

A total protein test is completed as part of your routine health checkup. It’s one of the tests that make up your comprehensive medical panel (CMP). It may be ordered if you have:

unexplained weight loss

fatigue

edema, which is swelling caused by extra fluid in your tissues

symptoms of kidney or liver disease

The total protein test measures the total amount of protein in your blood and specifically looks for the amount of albumin and globulin.

This test will also look at the ratio of albumin to globulin in your blood. This is known as the “A/G ratio.”

Elevated total protein may indicate:

inflammation or infections, such as viral hepatitis B or C, or HIV

bone marrow disorders, such as multiple myeloma or Waldenstrom’s disease

Low total protein may indicate:

bleeding

liver disorder

kidney disorder, such as a nephrotic disorder or glomerulonephritis

malnutrition

malabsorption conditions, such as celiac disease or inflammatory bowel disease

extensive burns

agammaglobulinemia, which is an inherited condition in which your blood doesn’t have enough of a type of globulin, affecting the strength of your immune system

inflammatory conditions

delayed post-surgery recovery

A/G ratio

Normally, the A/G (albumin to globulin) ratio is slightly higher than 1. If the ratio is too low or too high, additional testing must be done to determine the cause and diagnosis. If the ratio is low, it can suggest:

autoimmune disease

multiple myeloma

cirrhosis

kidney disease

A high A/G ratio can indicate genetic deficiencies or leukemia. Make sure to discuss your results with your doctor. They may want to do follow-up testing.

http://web.itu.edu.tr/~dulekgurgen/Proteins.pdf