Why the storage moduli E' decrease with highr fiber volume?

Hi guys, I am facing problems to interpret a DMA results. Material: Composite Bisphenol A + curaua fiber What is being tested: the influence of fiber volume on the composites properties...

12 February 2021 174 7 View

Which buffer should I use to aliquot antibody in?

for western blot analysis the buffer in which the antibody already is, written in the datasheet, or should I use already the bsa or milk solution that I use for final diluition?

17 November 2020 4,804 3 View

What is the best protocol for simultaneous extraction and purification of DNA and RNA from animal tissues?

I need to perform a co-extraction of DNA and RNA from pools of juvenile Daphnia magna. I used the AllPrep DNA/RNA Mini Kit (QIAGEN) but with poor results in terms of DNA yeld and quality. I would...

22 October 2020 9,746 3 View

No title

The threat of pandemic disease is not new: For decades, public health experts advised that a coronavirus outbreak was looming, global proportions, and that the world was not equipped to fight it...

27 May 2020 2,871 25 View

Is it correct to compare Fst values obtained from different genetic markers?

Fst is used in many genetic studies which involve different markers (mtDNA, microsatellites, SNPs...) as methodologies and next-generation genetics improve. I was wondering if it would be correct...

27 April 2020 7,175 3 View

My work entails comparing the effect of tax on economic growth of two countries. Asides the panel regression, which other technique can I use?

MY specific objective is to make comparative analysis on the effect of tax revenue on GDP of country A and country B

26 June 2019 6,619 3 View

In Morris water maze training results for scopolamine-injected rats, is it normal that scopolamine doesn't cause difference on day 5 ?

And if so, is it related to short term memory or inability of scopolamine to impair spatial learning?

17 June 2019 9,461 3 View

How to prepare aqueous solution of Methyl Jasmonate dissolved in Tween 20?

I need to prepare 500 mL of aqueous solution of 1mM Methyl Jasmonate dissolved in 0.1% of Tween 20 for foliar application. How much MJ and how much Tween 20 do I need to use? Both compounds are...

02 May 2019 6,228 2 View

May I express a protein in C. glutamicum using a commercialy availabe vector?

29 March 2019 7,700 1 View

How can i use ethidium bromide for sample staining?

I don't want to add it directly into the gel but just using it as loading dye.

25 March 2019 5,889 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View