What does " RSEM (Batch normalized from Illumina HiSeq_RNASeqV2)" in cBioportal mean?

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What are the "units" in RNA seq data processed by RSEM?

Hello! I've been using gene expression data from the TCGA, where the RNAseq is processed using RSEM. I did a few graphs such as boxplots showing the expression of different genes in normal vs...

14 October 2020 2,721 2 View

Which is best way to purify my His-tagged protein after eluting from a Ni NTA column in denaturing conditions (urea 8M)?

I have cloned two versions of my protein of interest to produce antibodies in mice. It is not soluble in native conditions, so I am trying to purify it in denaturing conditions with urea 8M. Now,...

18 February 2020 7,305 3 View

Multi-layer wells "robbed" of pumping rates?

By the way, I send attached the workflow I've performed. It is strange that in the Link to parameters window, in the Topology processing, instead of "no topology processing", now what I get is "no...

17 February 2020 8,662 3 View

How to reject authorship of some papers?

You added in my published paper list some article that don't belong to me. The author has the same family name as me, but is being active in a total different area of research. Thank you in advance

24 January 2019 5,795 3 View

Is there an easy way to count the diatoms in a rock sample in a short amount of time?

Hello! I have a bit of a struggle with my diatomites samples. I have tried all the methods to break the diatoms from the rock but I got a negative results (they are highly silicified) and I want...

04 September 2018 6,353 3 View

Any recommended (free) software to rectify and post-process coastal oblique images?

We have a fixed time-lapse camera (RGB) located on a hill oriented towards a sandy coastal area. Images are taken every 30 min. By using a set of ground control points (GPS) we aim to rectify and...

24 October 2017 4,632 5 View

Does Pfu Taq polymerase generate 5'P end?

Does the PCR product amplified using pfu polymerase have 5' phosphate groups to use for blunt cloning?

22 June 2016 1,058 3 View

How can I break the exopolysaccharide capsule of the mucous colonies to extract the RNA and DNA?

Hello to everyone! I'm working with strains of Acinetobacter baumannii resuspended in NaCl 0.45% (8 McF in 2 mL). Colonies of Acinetobacter baumannii are mucous so that they can not be separated...

15 June 2016 6,500 1 View

Why do I have a smear in gel for my bacterial RNA sample?

I have a question.....i have made a agarose gel of bacterial RNA and i have obtained a smear....what's happened? the RNA is 588mer. Moreover, i have done a Real Time PCR of these RNA without...

25 October 2015 7,189 4 View

Large-scale reaction with TnT T7 quick coupled transcription translation system (Promega)?

I wonder if the TnT T7 quick coupled transcription translation system (Promega) works in a large-scale reaction. They suggest a final volume of 50 ul and I would need a final volume reaction of...

17 August 2015 8,270 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View