Dear community:
Could anyone explain me what both means by the sample and reference energy on a 2487 Dual-Wavelength Absorbance Detector in HPLC? I mean, what does both the sample and reference energy represent in such a detector. Likewise, what should be the suitable difference between these two parameters in such a detector?
Thanks in advance!
William Letter
"Reference" will be the background signal generated from light passing through your mobile phase solution. "Sample" will be the signal generated from well, the "sample" in the flow cell.
- When you "Balance" or "Autozero" the detector, the "Reference" value is stored in the system and used as the baseline. Any change in signal from the "sample" passing through the flow cell generates a signal above or below this established "reference" level.
These two values allow us to generate ONE final signal, the output signal which is what is normally recorded.
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