I performed a cell cycle analysis using the cell cycle platform in FlowJo and found that some samples showed a negative value in the Sub-G0 population (see attached image). While I understand this result doesn’t make sense biologically, I’m wondering how I should interpret it and what steps I could take to correct these values. Thanks
Actually, the sub-G1 population is not well visible in the graph. Nevertheless, cells with sub-G1 DNA content may reflect cells that are undergoing apoptosis and have lost some of their DNA. Also, the standard evaluation of DNA content assumes that all cells have the same DNA content in G1, i.e. are diploid. In many cell lines, however, especially but not only cancer cells, this may not be the case.
when looking at the percentage of cells in G1, G2 and S, you got a total of 104.9 %. Therefore the machine indicates - 4.8 % in less G1 simply to have a final count of 100%. But here I don't think that this last percentage (- 4.08 %) corresponds to real apoptotic cells or cell debris. You have probably to see with the operator of the machine to undersatnd this point.
Wolfgang A Schulz thanks for your reply. I meant the negative value of that population. Makes no sense.
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