Dear Investigator,
The comet assay has been in existence for many years, but the general protocol largely remains unchanged, despite it requiring numerous, time-consuming steps. We in the Oxidative Stress Group, at Florida International University, are very interested in learning about comet assay users’ experiences, and their thoughts on the protocol. Below is a link to a short questionnaire that we would be grateful if you would complete. All replies are entirely anonymous, but you will have the opportunity be entered into a prize draw for an Amazon voucher.
https://fiu.qualtrics.com/jfe/form/SV_dh9MYqcgHp42LoV
(you might need to cut and paste into browser.)
Feel free to distribute to other colleagues who may be interested.
Please feel free to contact any of us if you have any questions, or want to confirm the veracity of this email and questionnaire.
Thank you very much in advance,
Marcus
Professor and Head of Department.
Oxidative Stress Group, Dept. Environmental & Occupational Health,
Florida International University, Miami, FL 33199.
From my perspective this is a very promising assay. However 98% of the execution is done badly, resulting in wrong non-reproducible results. More often than not these results are affected by the assay itself and not solely by the underlying biological effects.
But these effects can be avoided through proper lab independent standardization technology like the 4D Lifetank. Only this will allow for further use of this assay as a diagnostic tool.
quality of comet test required experience because most of test is hand work. is give a good result if combine with other cytogenetic tests
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