I just read about the Li-Cor 4300 Genetic Analyzer. As I understand it it is a system for DNA analysis which uses fluorescent labeling in the Infrared zone of the spectrum. Plus, it uses gel electrophoresis instead of capillary electrophoresis. Have someone used this system? What are your oppinions on it? Have anyone used both, this and a capillary system (i.e. applied biosystems), so they can do a fair comparison?
I used the Licor 4300 for DNA electrophoresis (TILLING method), and it was working quite well, the PCR product was cut by enzyme in the site of mutations and based on the image gel we detected in which sample the mutation was present. The primers used for PCR were fluorescent labelled. I don't know if for protein studies this system is also fine. Capillary electrophoresis in my lab we are using for protein separation, but right now we have a lot of problems with machine, mostly the time the capillary is broken or something is wrong with the machine. So I can't say if that is better option, because right now it is difficult to obtaining any repeatable results for CE.
Hi Magdalena. Protein analysis by CE is one of the routine assays used for quality control in the Pharmaceutical industry. Please let me know if you need some help.
Am also looking for an answer. My results with the same DNA samples and SSR primers yield different results in LICOR and ABI capillary.
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