Hello everyone,
I renovate the cell list for the cells we store in -196 liquid nitrogen tank in our lab and I wonder what you include in your list for this. Cell name, passage number and the date I passage and store them in the tank are my elements for the list. What would you add beside these?
Thank you.
Hello Emre Tali,
Besides cell name, passage number and date, you may add the freezing medium composition and the number of cells frozen per vial.
Best.
Who the cells belong to and any identification number (hospital number or similar) and what culture medium is used for each line and possibly any special hazards if your lines express viruses . Date checked for mycoplasma contamination and whether the line has been checked for purity ( no contaminating HeLa cells etc)
+1 for adding the name of the person freezing and the cell count on freezing