Is chromatin intact in tissues stored in RNAlater?

More Dhanya Ramakrishna Iyer's questions See All

Anyone has an experience extracting proteins from rectal swab samples?

I am working with rectal swab samples in the field of colorectal cancer research. Does anyone have experience (or can point towards any relevant literature) of specifically working with proteins...

02 February 2021 3,622 3 View

How do you check the occupied d-orbitals in VASP?

I am running VASP calculations involving transition metal atoms in which there are multiple unpaired electrons in the d-orbitals but I am not sure how to check the individual d-orbitals (ex:...

24 August 2020 1,947 2 View

Can you check spin multiplicity on individual atoms in VASP?

I am doing VASP calculations with a bulk triclinic lattice system involving transition metals Cr and V. I know that you can check the spin for the entire system with the OSZICAR file from VASP but...

16 July 2020 9,052 3 View

How to make a scatter plot for many variables and 2 groups in one graph?

I have 2 groups and have 7 variables. I would like to make a stacked scatter graph to present it all in one graph. Is it possible and how?

13 July 2020 2,145 3 View

Beta value calculation of absolute intracellular calcium concentrations?

Hi all, I am doing calcium imaging experiments in N2a cells and wanted to calculate the absolute calcium concentrations. Can someone help me out in figuring out how to calculate the fluorescence...

24 March 2020 7,974 1 View

Workfunction from LOCPOT?

Does anyone know how to derive the work function from the LOCPOT file from vasp?

24 March 2020 851 5 View

I have 2 groups to compare by removing the effects of age and the data is not normal. How do I do so in SPSS?

The 2 groups are parents and their children. The data is not normal. Would like the comparison without the effects of age.

21 February 2020 7,714 4 View

Hi, I have child-mother data on a quantitative trait and need to know how to calculate their heritability?

I have done a spearman correlation as the data is not normally distributed and now would want heritability index...am I correct in looking at heritability with this data? If yes, how do I go about?

14 February 2020 9,160 3 View

Experimental method for determination of C-H (homolytic) BDE in organic compounds?

Hello Everyone, We want to measure the C-H (homolytic) bond dissociation energy of some organic compounds. Any practically feasible tool in the common analytical laboratory that can give us the...

13 February 2020 7,588 2 View

How to decide on which MDR strain (GN bacterium) to buy from ATCC?

I want to purchase well characterized MDR strains of a Gram-negative bacterium from ATCC to look at modes of resistance. I am also going through literature to see if people have purchased a...

05 June 2019 7,721 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Andrea Cerase

Most likely you can't! I would never trust the results you get anyway..

Bornika Roy

There's a PLoS One paper where ChIP was performed on RNALater-preserved tissue (Article Chromatin immunoprecipitation (ChIP) method for non-model fr...

) and since it is advertised as a preserving agent for DNA and proteins as well (https://www.researchgate.net/post/Western-blots-on-tissue-which-have-been-stored-in-RNAlater) I think it might work if your samples aren't too old