9 Questions 9 Answers 0 Followers
Questions related from Johan Ariff Mohtar
Hi, I am facing a problem in screening the normal silk gene (476 bp) in wild type and Cas9 mutant samples (Cas9 targeting the 476 bp gene) using high fidelity polymerases in the PCR. As you can...
01 January 2023 9,752 0 View
Hi all, I would like to ask, if two sgRNAs are mixed together at fixed ratio without Cas9 enzyme, say 1:1, how long can they be stored at -20 degrees before use? Should they be used immediately...
20 October 2022 9,802 0 View
I would like to ask about the depth of analysis for producing a paper on identification of species using DNA barcoding technique. For example, I have sampled 3 species of spiders in cave and...
10 October 2022 5,160 4 View
Hi, I have been searching papers that indicate a full/complete solubility of native spider web in chemical solutions such hexfluoroisopropanol or lithium bromide but could not find any. I have...
04 November 2017 2,734 1 View
I want to know the general steps to study gene promoter using CRISPR CAS9 in insects. A new study Wei et al., 2014 has successfully employed the technique to study heritable gene modification in...
19 June 2017 525 2 View
No contamination occurred so far.
18 November 2016 3,365 4 View
Reasons??
08 October 2016 5,180 2 View
Hi, I am dissolving spider silk fibre in Ajisawa solution and I am preparing the solution for the experiment. However, I am pretty not sure the way to prepare this solution (molar ratio 1:8:2,...
20 July 2016 7,930 2 View
Hi, I am going to dissolve silk fibers from spider using 75% (w/v) calcium nitrate-methanol solvent. However, I am not sure the purity of methanol should be used. Can anyone help me on this...
18 July 2016 4,155 1 View
