Johan Ariff Mohtar

6 Questions 6 Answers 0 Followers

Questions related from Johan Ariff Mohtar

Why cant high fidelity polymerase amplify the Cas9 mutant gene?

Hi, I am facing a problem in screening the normal silk gene (476 bp) in wild type and Cas9 mutant samples (Cas9 targeting the 476 bp gene) using high fidelity polymerases in the PCR. As you can...

01 January 2023 9,700 0 View

Mixing of Two sgRNAs?

Hi all, I would like to ask, if two sgRNAs are mixed together at fixed ratio without Cas9 enzyme, say 1:1, how long can they be stored at -20 degrees before use? Should they be used immediately...

20 October 2022 9,741 0 View

The Depth of Analysis for DNA Barcoding Paper?

I would like to ask about the depth of analysis for producing a paper on identification of species using DNA barcoding technique. For example, I have sampled 3 species of spiders in cave and...

10 October 2022 5,083 4 View

What could have happened to my spidroin@silk fibre protein (180-350kDa) after filtration with 0.2 um membrane? Not detected on the FPLC chromatogram?

Reasons??

08 October 2016 5,092 2 View

How to prepare Ajisawa solution the right way?

Hi, I am dissolving spider silk fibre in Ajisawa solution and I am preparing the solution for the experiment. However, I am pretty not sure the way to prepare this solution (molar ratio 1:8:2,...

20 July 2016 7,878 2 View

Preparation of 75% (w/v) calcium nitrate-methanol system?

Hi, I am going to dissolve silk fibers from spider using 75% (w/v) calcium nitrate-methanol solvent. However, I am not sure the purity of methanol should be used. Can anyone help me on this...

18 July 2016 4,117 1 View