I tried doing a qpcr analysis after the whole process I got no Ct value everything, washing negative on the analysis table.
There could be various reasons for not having a CT value after a qPCR analysis. Here are some possibilities:
No amplification: The qPCR reaction may not have amplified any target DNA/RNA, in which case a CT value would not be produced.
Insufficient starting material: The amount or quality of the starting material (DNA/RNA) may not have been sufficient to produce an amplification signal.
Inhibition: The qPCR reaction may have been inhibited by substances in the sample or from the reaction components, preventing amplification.
Technical issues: There could be issues with the instrument or reagents used for the qPCR, such as incorrect temperature or timing settings or contaminated reagents.
Poor primer/probe design: The primers/probe used for the qPCR reaction may not be specific enough or may not work well with the sample being tested, causing amplification failure.
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