I used linearized plasmid DNA to create qPCR standard curve. Taqman qPCR showed efficency around 95%, and sensivity is 10 copy per reaction. However, when I spike this standard curve with 100ng PBMC genomic DNA, the sensitivity is gone. Signal lost from 1000 copy. The target gene has been blast with humand genome do not have any binding. Dose anybody know where this problem could come from?

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