Racoco Duan

2 Questions 3 Answers 0 Followers

Questions related from Racoco Duan

What could be the reason of genomic background DNA mass up the qPCR standard curve?

I used linearized plasmid DNA to create qPCR standard curve. Taqman qPCR showed efficency around 95%, and sensivity is 10 copy per reaction. However, when I spike this standard curve with 100ng...

03 March 2020 7,750 3 View

Why my qPCR negtive controls sometimes show two phase amplication curves?

Could anybody help me with my qPCR issue? I have cellular gDNA from human PBMCs serve as my target gene negative control. I did blast and in silico test, my qPCR product do not have any match with...

01 January 2020 7,246 4 View