I conducted a research on heavy metals accumulation in crops and animal (Bull, Cow, Goats, Buffalo's) hair... after analysis with atomic absorption spectro photometer I found that copper gave awkward result as no concentration was detected while some of the samples gave reading in negative values. However, cadmium was found in toxic range. The hair washing was done by the method recommended by IAEA and digestion was done with HNO3+H2SO4+HClO4 (8:1:1). I'm looking for comments from experts of this area of interest, regarding copper deficiency in animals. what could be the possible reasons.
In non pigmented hair samples there is nearly zero concentration of copper, it is normal.
Use pigmented hair, black or red.
Your concentrations would be below the detection limit, not necessarily a "true" zero. Negative values: I would double check the (dry) weigh of your samples used to calculate the concentrations.
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