Hi all,

I am planning to perform Gelatin Zymography using conditioned media from three different knock out from same cell line. I seeded equal number of cells and collected the conditioned media (serum free) for Gelatin Zymography. Is there any way I can address loading control in this assay. Do people normally quantify protein concentration from the collected conditioned media before loading it into Gel? even if we quantify by BCA, how would I show it in the final figure?

Also, the total volume of my conditioned media is roughly 500 ul. Do we need to concentrate it to, lets say 100 ul, across the set and load equal volume if we do not quantify protein concentration?