How to remove straight line from mosaic Landsat image?

I have used mosaic to new raster tool to join two aerial image from Landsat 8 of different path. However, raster files had color variation and after mosaic I got a straight line running from...

22 December 2020 4,640 4 View

How to define population proportion (prevalence) for sample size determination?

How to define population proportion (prevalence) for sample size determination? We often see the equation as: Sample Size= Z2.p.(1-p)/d2 in this particular sample size determination equation which...

12 October 2020 2,421 4 View

How to choose a scientifically correct sample size?

I have been studying and working on research methodology for at least five to six years but still I could not satisfy my self with the answers and references provided in the books and web...

06 October 2020 491 21 View

What is the ideal Elution buffer composition for a His tag protein purification to prevent protein aggregation after freeze thaw ?

Hi All, Recently, I purified 8 proteins and stored at -20 with the elution buffer containing 20 mM Tris, 250 mM NaCl and 200 mM Imidazole. I autoclaved the buffer, so I do not know whether the...

25 September 2020 9,814 6 View

How many washes with regeneration buffer are enough for a 10 ml column containing Ni resin?

Hi All, We recently used HisPur-NiNTA resin for protein purification. The protocol from Thermo says that 20 mM MES,100 mM NaCl, pH 5 is required to regenerate beads without affecting Ni content....

14 August 2020 4,083 3 View

Tools to simulate attacks on IoT networks for educational purpose?

Hi What are the best simulators/emulators that provides a wide support to simulate attacks on iot devices and protocols (BLE, MQTT, authentication, MITM, ddos, sybil ...) and that offers by the...

15 April 2020 355 2 View

Is there anyone who can guide me for analyzing the Uncertainty Quantification of numerical models using ANSYS fluent and MATLAB?

I am facing problem while writing the journal file in Fluent using TUI commands. I could not extract result ( temperature distribution along the center line, velocity distribution along the...

31 August 2019 5,806 3 View

Max extension time for pcr with Phusion Hot start II DNA polymerase?

Hi all, I am planning to PCR a 7430 bp fragment from a big plasmid(11 kb) with Thermo Phusion hot start II DNA pol. Thermo recommend to use 15s-30s as extension time per 1 kb length. Overall GC%...

08 August 2019 7,405 3 View

How efficient and good is NEB Q5 Site Directed Mutagenesis kit?

Hello All, I just want to know your thoughts on NEB Q5 site directed Mutagenesis kit. Has anyone used this ? Is it good ? I have a plasmid of 6 kb on which I design my SDM . Your advice and...

18 June 2019 1,585 7 View

What is the maximum insert length limit for ligation?

Hi All, I am planning to clone one of my inserts into VC155 BiFC plasmid. My insert size is 8 kb where vector size is 5kb. Is it very hard to clone 8 kb into 5 kb? Also I would like to do...

21 May 2019 7,011 3 View

How to match the XRD of a compound for which there is no JCPDS file ? Is there any other characterization to claim the synthesis of desired compound?

I have synthesized LiMgF3 using the starting precursors LiF and MgF2 via solid state diffusion method. Now I am looking for some characterization to claim the desired product, as there is no...

09 February 2021 921 3 View

Difference between Tesla Model 3 LFP vs. LCA?

What are the technical differences between the Model 3 with LFP vs. LCA cells. What does the customer feel as the difference?

01 January 2021 1,180 1 View

Asked a question in SnO2 ?

08 December 2020 6,957 2 View

Why is the impedance of ECoG electrodes tested at a frequency range from 1 Hz -100 kHz?

I am testing the impedance of a material that will be used for ECoG electrodes. I was told to test it at a frequency range from 1 Hz - 100 kHz but why is this range used? I wasnt able to find a...

08 November 2020 8,089 3 View

Why in some papers people have shown one jcpds card no. for two different metals?

most people have shown I their research articles that the intensity peaks of different metals like ag, au and even pd have their peaks on the same degrees i.e. nearby 38.2, 44.2,64.6, 77.5 degrees...

05 August 2020 7,751 4 View

Can anyone provide me all the reference JCPDS cards for ag, au and pd?

I need these reference cards to match them with my data. if you can help then kindly provide the pdf files or even images for each card. I do have one reference card for each but it lefts me the...

05 August 2020 6,147 4 View

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01 February 2020 1,696 1 View

Please , I need (XRD) data sheet standard (JCPDS manual). for ( hydroxyapatite, multi-walled carbon nanotubes, High-density polyethylene ) materials?

XRD data sheet standard JCPDS manual

01 February 2020 7,225 3 View

I need JCPDS file for Ag, Au, Pt and Pd Nanoparticles (NPs were synthesized using biomaterials). Can you help me please?

11 December 2019 793 4 View

Where can I find an online resource to get XRD JCPDS files?

We are looking for to find xray card for my organic dyes to specified Miller indices present in XRD powder e.g (c39H44N606)(C18H26N9O6) Best regards and thank you very much for your help and...

12 November 2019 8,699 2 View