What concentration of PBS could be used to lyse cells?

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Trizol can be used for the storage of tissue sample?

Can I store the freshly collected tissue sample in trizol instead of RNA later buffer? if Yes, How long can i store? will it affect on quality/quantity of the RNA sample? Which is better among...

02 June 2024 582 3 View

Lactococci growing on MRS media?

Dear all, I need help regarding my research on isolation and screening of potent LAB producing antimicrobial substances against skin pathogens. Few isolates obtained from different milk sources,...

16 May 2024 8,126 0 View

Is there any book relevant to queueing with inventory?

Because of my research area comes under this topic, so I want to study more information about this content.

11 April 2022 4,398 4 View

Can we consider hemagglutination assay for dengue virus as a quantitative method???

For a pure virus, HA can be considered as a way of representing their titre. even though it is cost effective and less labor intensive, it is less concerned. Kindly guide me in this matter.

04 July 2019 4,055 1 View

How to create cylinder sector mesh for ANSYS Forte combustion and emission analysis?

Ansys Forte analysis for RCCI engine

12 June 2019 3,526 0 View

Does body mass index correlate with breast cancer incidence?

community based screening for breast cancer in obese persons

19 September 2017 7,586 3 View

How different is western blot protocol for phosphorylated protein from regular western blot?

Hello everyone, I am trying to probe for phosphorylated ERKthrough western blot. How different is the protocol from regular western blot? I looked up on abcam and they ask to use bsa instead of...

30 August 2017 2,797 9 View

Can anyone give me a protocol for stable luciferase transfection of murine cell lines?

I want to do a stable luciferase transfection of murine PDAC (PANC02) and colorectal (CT26) cell lines. Please help me find where to obtain the protocol and reagents. Thank you. Sincerely, Lakshmi.

05 April 2017 2,610 4 View

Whats is the maximum concentration of NaOH that is not toxic to cell cultures?

I am using 1M NaOH solution to dissolve a drug. I am trying to estimate its IC50 using MTT assay. The data sheet says that the drug is soluble in 1M Naoh at 50mg/ml. Has anyone had experience...

18 September 2016 1,182 6 View

If anyone has done GCMS for tetrodotoxin detection?

If anyone has done GCMS for tetrodotoxin detection, please help me to know the compound names for the characteristic fragments of 2-amino 6-hydroxymethyl 8 hydroxyquinazoline that forms peaks at...

02 September 2016 3,328 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View