Can we consider hemagglutination assay for dengue virus as a quantitative method???

For a pure virus, HA can be considered as a way of representing their titre. even though it is cost effective and less labor intensive, it is less concerned. Kindly guide me in this matter.

04 July 2019 3,881 1 View

How different is western blot protocol for phosphorylated protein from regular western blot?

30 August 2017 2,594 9 View

Can anyone give me a protocol for stable luciferase transfection of murine cell lines?

I want to do a stable luciferase transfection of murine PDAC (PANC02) and colorectal (CT26) cell lines. Please help me find where to obtain the protocol and reagents. Thank you. Sincerely, Lakshmi.

05 April 2017 2,462 4 View

Whats is the maximum concentration of NaOH that is not toxic to cell cultures?

18 September 2016 998 6 View

If anyone has done GCMS for tetrodotoxin detection?

If anyone has done GCMS for tetrodotoxin detection, please help me to know the compound names for the characteristic fragments of 2-amino 6-hydroxymethyl 8 hydroxyquinazoline that forms peaks at...

02 September 2016 3,180 2 View

I just want to know factors affecting crystallite size of a materials?

01 April 2016 1,566 13 View

Can someone suggest protocols for assaying catalase, glutathione peroxidase, superoxide dismutase and lipid peroxidase activities in animal tissues?

18 September 2014 2,279 4 View

Can anyone tell me what will happen if you keep your protein precipitated in Methanol/0.1MAmmonium acetate for more than a week?

Isolation of protein from plant leaves.

30 September 2013 4,149 1 View

Will the RIN value decrease if RNA is isolated from FFPE Blocks??

As I need to isolate RNA from FFPE blocks using kit, i need to know the valuable answers before I start with the procedure. Pls share your experience if any

01 January 1970 335 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View