Indumathi Mahadevan

10 Questions 5 Answers 0 Followers

Questions related from Indumathi Mahadevan

If anyone has done GCMS for tetrodotoxin detection?

If anyone has done GCMS for tetrodotoxin detection, please help me to know the compound names for the characteristic fragments of 2-amino 6-hydroxymethyl 8 hydroxyquinazoline that forms peaks at...

03 September 2016 3,359 2 View

Can anyone help me troubleshoot problems in sample derivatization in GC-MS?

My processed fish extract samples is evaporated to dryness and a residue is obtained which is not getting dissolved in MSTFA+TMCS silylating reagent. I am worried if the problem is in the final...

09 August 2016 3,158 6 View

Can anyone suggest me a derivatizing method for my fish extracts?

I am working on puffers and I have to prove the presence of Tetrodotoxin in them through GC-MS. I processed my fish extract with a standard procedure, and in that I have to evaporate the final...

21 July 2016 6,143 2 View

Requisition for MSTFA

I am working on puffer toxins and I need to detect the presence of Tetrodotoxin in crude fish extracts. So I need to derivatize my samples before GC-MS. I need 1 ml of MSTFA reagent...

06 April 2016 265 1 View

Is the use of Trimethylsilyl compounds necessary for GC-MS analysis?

I am working on subcellular origin of puffer fish toxins and I need to detect the presence of Tetrodotoxin in crude tissue extracts of the fish through GC-MS. Is it mandatory to make...

31 March 2016 2,127 2 View

What concentration of PBS could be used to lyse cells?

I am working on subcellular localization of toxin in fish tissues. So what concentration of Phosphate buffered saline be used to lyse the cells during homogenization

11 March 2016 9,606 3 View

Can anyone give me simpler protocols for measurement of oxidative stress in animal tissues?

I need to analyse the oxidative stress of my mice tissue samples. So I need standard simple methods to perform it. I need to analyse the following enzymes, viz, Superoxide dismutase, Glutathione...

12 February 2015 6,729 6 View

studies on bacterial origin of tetrodotoxin from puffer fish

I need to study the bacterial origin of tetrodotoxin from my puffer fish. When I am isolating different cultures from the puffer fish, can I directly inject the supernatants of the bacterial...

01 October 2014 6,753 4 View

Can someone suggest protocols for assaying catalase, glutathione peroxidase, superoxide dismutase and lipid peroxidase activities in animal tissues?

Enzymatic assays for animal tissues. But an alternative to gel method. I could'nt find the protocols online. So please help me out in this.

19 September 2014 2,440 4 View

What are the normal human cell lines most suitable for toxicity studies?

I need to test the toxicity of my fish extracts on human cell lines. I would like to know the normal human cell lines most suitable and available for toxicity testing other than suspension...

16 September 2014 5,357 1 View