How to check the radiation characteristics of IR thermal emitters using COMSOL?

I have designed a MEMS-based IR thermal emitter and done the electro-thermal simulation to get the temperature profile of micro-heater. Now I want to check the radiation intensity vs wavelength...

14 October 2020 4,542 2 View

MaxQuant Label free Data analysis - Is it right approach to first log2 transform LFQ intensity and then Normalised before taking a ratio?

I have a three biological replicates of treatment and Control samples. I am using MaxQuant for the data analysis . Currently, LFQ intensity has been log2 transform and then normalized....

30 October 2019 8,380 1 View

In Proteomics studies, can we used emPAI values to do Label free based proteomic quantitations and is there good reference to follow?

emPAI based label free quantitation

07 April 2019 2,818 3 View

Please tell me what is better subroutine or python script for simulation of grain and grain boundaries on ABAQUS?

Also if possible please suggest me from where I can learn to write accurate subroutine for microstructure simulation because I am new on subroutine writing.

09 August 2018 6,126 1 View

How can I avoid E. Coli adherence to glass cover slips ?

I am imaging very high concentration of E. coli solutions under confocal microscope. However, the bacterial cells always stick to the cover slip. Is there a coating which prevents their sticking ?...

07 July 2018 6,407 1 View

How to model Resin flow for composites in Abaqus/ Msc. Patran?

10 May 2017 9,267 1 View

Can someone help me prove the approximate coefficient of numerical diffusion?

Hi, Can someone please help me with deriving that false/numerical/artificial diffusion is flow direction dependent and it is given as (image attached). also as per Patankar book , numerical...

30 October 2016 6,390 11 View

Has any one doing western blot for acetylated p53 (K382) in SHSY5Y cell?

12 October 2016 357 5 View

If Turbulent flows are characterized by high Reynolds number, why they are said to be highly diffusive in nature ?

Hi, since the Reynolds number is high for Turbulent flows, the viscous force are over powered by convective forces, meaning the dampening of disturbances will not be efficient. This results into...

27 July 2016 5,758 8 View

Why are JC1 dye monomers and dimers excited at two different wavelengths?

JC-1 dye monomers are excited at lower wavelengths and emit green, while the aggregates are excited at a higher wavelength and emit red. Why is this? Why do aggregates absorb at a higher...

13 June 2016 8,675 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View