I am using FM4-64 FX to stain Klebsiella bacterial cells.
1 ug/ml is a good concentration for E. coli. I think it is also suitable for other Gram-negative bacteria.
I also feel that 1 to at the most 2ppm should be sufficient. You need not do the gram staining.
Just want an information about taxonomic changes in AMF
19 October 2023 2,971 0 View
. vno file to excel file.
17 September 2023 3,788 0 View
what makes the difference if we choose metal chlorides or acetates instead of metal nitrates for synthesizing MOFs
15 May 2023 1,652 3 View
From the adsorption studies at different temperatures, i have calculated the heat of adsorption for the adsorbent. Using this value, how can we calculate the regeneration temperature? or can we...
17 April 2023 4,318 4 View
How can we analyze adsorptive sites for CO2 in MOF by doing FTIR of a sample after CO2 adsorption?
06 April 2023 2,222 0 View
My XPS spectra of Nickel 2p of MOF is showing huge noise. What could be the reason for this?
03 April 2023 5,750 3 View
Which solvent is used to disperse HKUST MOF for UV-Visible spectroscopy and particle size analysis?
03 April 2023 4,403 4 View
We work on primary Cancer cell derivation from patient samples so i want to immortalize a cell type can anyone suggest lentriviral protocol
22 February 2023 6,704 2 View
I have followed some research paper and found that some plants having root nodules shows hyphal infection but do not shows vesicles ant arbuscules. What is the reason behind it.
02 May 2022 6,399 3 View
I want to simulate internal flushing flow like the dielectric will flow internally through the electrode. Please help me with the simulation parameters.
09 December 2021 9,659 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
