How to collapse the membrane voltage of E. coli with valinomycin? What kind of condition this chemical needs? High K+ concentration?

I try to use valinomycin to collapse the membrane voltage of E. coli but fail. I have try the environment of different K+ concentration (from 100mM to 400mM). The valinomycin concentration I use...

28 January 2016 5,832 4 View