In my experiments, I found that different monoclonal cell lines from the same host cell produce similar concentrations of ammonium ion when using the same medium; but when the same monoclonal cell line cultured with different types of fermentation medium, the concentrations of ammonium ion vary greatly. Is it because which of the ingredients in the medium is different that makes this difference?
Hello Xukun Xu
The components in the medium affect the concentration of ammonium ions. L-Glutamine is the source of nascent ammonia, either through its spontaneous degradation or L--Glutamine metabolism. Also, L-Glutamine decomposition over time, depending on the storage temperature, is the principal determinator of the L-Glutamine decomposition rate. L-Glutamine is so labile, that it is often omitted from commercial liquid medium preparations to lengthen the product shelf life. Thus, to minimize both lactic acid and ammonium production, Glucose as well as L-Glutamine may need to be simultaneously controlled or replaced. You may refer to the article attached for more information on this subject.
Article Effects of amino acid addition of ammonium stressed CHO cells
Best.
- Badges
- Science method