What does it mean if in mediation analysis the indirect effect is significant but is a larger b value than that of the direct effect?

I understand that for partial mediation to have occured the CI for the indirect effect must not cross zero and the b value for the indirect effect should be smaller than the b value for the direct...

14 February 2021 3,273 7 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View

Is there a solution to this conundrum on freefall into a black hole?

18 January 2021 1,582 3 View

Lipid Force Field with gromacs command pdb2gmx?

I have a pdb file that contains DPPC molecules and other molecules. I want to generate .gro and .top files from this pdb file using the Gromacs command pdb2gmx. I am trying to use the Charmm36...

08 January 2021 3,751 6 View

How do I compare 10 groups of people in age brackets?

I am looking at age of people in 10 distinct groups. The number of persons in each age bracket is noted and there are 10 age brackets. The total number in each group is quite varied but data is...

12 June 2020 5,056 3 View

What sample size do I need if my total population is 195 (countries in the world)?

Hi everyone, (Please excuse the ambiguity within the question - protecting my idea) I recently discovered some indexes which I and colleagues would like to see if scores on these indexes across...

02 June 2020 3,960 11 View

How do you report a three-way ANOVA with robust standard errors from SPSS output?

Hi everyone, I need some help--and I'm hoping that all of you who are better at statistics than me will be able to help the fog lift! Background to my problem: I have done a three-way ANOVA...

19 May 2020 2,103 4 View

When do I remove participants instead of impute data?

I have a data set of around 1630. Some variables are missing in 20% of the sample (the later questions - survey fatigue/drop out). (I'm considering removing 250 participants who didn't answer...

17 March 2020 7,667 5 View

Does anyone know of papers detailing hand-back criteria for P3 infrastructure projects?

Hand-back criteria are those metrics used to measure the condition of a facility before it is transferred back to the owner/client at the conclusion of the concession period.

14 November 2019 8,998 2 View

Will we have a problem with water-soluble polymers as used in , for example, dish washer tablets?

What is known about the degradation of such polymers in the environment?

07 July 2019 3,363 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View