What common false positive proteins are there in affinity purifcation and mass spectrometry?

04 April 2014 1 9K Report

I have recently done affinity purification and mass spectrometry. The aim is to identify new protein interactors of the protein of interest.

I have the MS result from the first trial, where I found a lot of heat shock protein, which is likely false positive. I wonder if there are any other proteins that I need to pay attention to?

John S Wishnok

Here's another useful resource (generally detected proteins):

http://onlinelibrary.wiley.com/doi/10.1002/pmic.200800826/abstract

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