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Questions related from KC Lee
I am looking for sandwich Elisa kits for human CTNND1, and I have found the kits from the above companies. However, our group are not familiar with these companies, and I cannot find publications...
02 February 2020 9,011 1 View
My experiment is isolating primary cells from biopsy of different patients. And then to culture the primary cells in two conditions, one is the negative control, and the experimental set-up is...
07 July 2019 6,121 6 View
I am new to research of miRNA and I have been asked to do a knockdown on a miRNA by miRNA sponge technology. However, I am still confused about the maturation process of miRNA. According to figure...
02 February 2015 6,226 1 View
I have recently done affinity purification and mass spectrometry. The aim is to identify new protein interactors of the protein of interest. I have the MS result from the first trial, where I...
04 April 2014 8,622 1 View
I am using the recipe of mild strip buffer suggested by abcam. http://www.abcam.com/ps/pdf/protocols/Stripping%20for%20reprobing.pdf It works fine but after one month, the solution turned milky,...
09 September 2013 1,018 4 View
I am going to do the fractionation of cell lysate into the nuclear fraction and cytoplasmic fraction. The cell line I use is HEK293T. I want to know whether the fractionation is efficient by...
04 April 2013 8,704 1 View
I am going to do western blot for the nuclear fraction of mouse embryonic stem cells (E14 cell line). What are the nuclear loading controls that are commonly used? I know that Lamin B1 is a common...
04 April 2013 687 2 View
I am a beginner in western blot. Sometimes I need to store the PVDF membrane for later re-probing. I have asked my colleagues, some said I should dry the membrane and store it in 4 degree but I...
03 March 2012 2,835 39 View
