Has anybody used the Elisa kits from Elisa Genie, LifeSpan BioSciences, Aviva Systems Biology, Abbexa and Antiboides-online?

I am looking for sandwich Elisa kits for human CTNND1, and I have found the kits from the above companies. However, our group are not familiar with these companies, and I cannot find publications...

01 February 2020 8,911 1 View

Rules for paired sample t-test with samples from cell culture?

My experiment is isolating primary cells from biopsy of different patients. And then to culture the primary cells in two conditions, one is the negative control, and the experimental set-up is...

06 July 2019 5,986 6 View

Will both 5p and 3p strands become the mature miRNA?

I am new to research of miRNA and I have been asked to do a knockdown on a miRNA by miRNA sponge technology. However, I am still confused about the maturation process of miRNA. According to figure...

01 February 2015 6,127 1 View

What common false positive proteins are there in affinity purifcation and mass spectrometry?

I have recently done affinity purification and mass spectrometry. The aim is to identify new protein interactors of the protein of interest. I have the MS result from the first trial, where I...

03 April 2014 8,533 1 View

Strip Buffer Turns Milky?

I am using the recipe of mild strip buffer suggested by abcam. http://www.abcam.com/ps/pdf/protocols/Stripping%20for%20reprobing.pdf It works fine but after one month, the solution turned milky,...

08 September 2013 616 4 View

What is the nuclear loading control for embryonic stem cells?

I am going to do western blot for the nuclear fraction of mouse embryonic stem cells (E14 cell line). What are the nuclear loading controls that are commonly used? I know that Lamin B1 is a common...

03 April 2013 594 2 View

How to store a membrane in Western Blot?

I am a beginner in western blot. Sometimes I need to store the PVDF membrane for later re-probing. I have asked my colleagues, some said I should dry the membrane and store it in 4 degree but I...

02 March 2012 2,274 39 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View