Hi! For reprogramming PBMCs into iPSCs, I understand that you first must obtain the PBMCs by a density gradient, such as Ficoll-Paque, and then you have a product of primarily lymphocytes with various cell types (T cells, B cells, etc.). I was wondering if it is known which cell types in particular are ultimately the ones that reprogram? And why during the expansion process of the PBMCs using the CytoTune kit do I not have to add some type of antibody to create T cell activation... or is that activation occurring from a component in the media that is proprietary and unknown? Hope this makes sense :)
I was asked this during my preliminary exam and I told the committee member that only cytokines are listed as an addition in the media, but no antibody-- this leads me to think that T cells are not the target for reprogramming. I've gone down a rabbit hole in the literature and only came out more confused.
- Badges
- Science topic