What cause peak of l-lysine HCl to appear broad and how can i treat it?

Medhat Emad @Medhat_Emad2

05 May 2018 0 555 Report

I have a problem with l-lysine HCl peak in sample and standard as it appear with shoulder or broad.

Badges
Science method

More Medhat Emad's questions See All

Can any one help me with an hplc UV method for determination of D-Sorbitol?

I am looking for a method to determine D-Sorbitol using hplc UV detector.any one can help

04 May 2018 9,054 7 View

What cause the sample and standard Peak to have a negative absorbance?

I have a problem in hplc analysis of L-cystine analysis that the peak of both sample and standard have negative absorbance. This the first trial . -UV detector at 500nm. -Mobile phase phosphate...

11 December 2017 8,108 2 View

In dissolution test for tablets, What kind of filters used to filter samples contain indigo carmine aluminum lake (E132) as colorant?

In dissolution test for Abilify 5mg (aripiprazole) Results show high results about 110%, while maximum result should not exceed 104%(max. result in content uniformity test). This problem does not...

07 August 2017 7,559 2 View

What is meant by HPLC grade water?

We have RO station produce purified water of conductivity less than 1.5µs/cm. will be any problem if i use it for HPLC analysis after filter it through 0.22 micron filter

31 December 2016 2,693 4 View

What does it mean by about if it said in a monograph that the retention time is about 20?

In a monograph ,it said that the retention time of the peak is about 20.

10 November 2016 2,467 6 View

What cause shifting of retention time of a peak forward?

1- I have problem in shifting of retention time of a peak : During analysis of rebamipide, the peak of rebamipide shift forward. for example(Std at 20.0 and sample at 20.5), and as the sample set...

10 November 2016 1,481 10 View

Why does the peak of one substance disappear when injecting a mix of two, but when I inject each substance alone separately, they both give peaks ?

mix consists of propylparaben as internal standard and glycyrrhizic acid, when i inject the mix only peak of internal std appear but when i inject each one alone each peak appear. using HPLC.my...

11 December 2014 9,946 13 View

How can I determine the shelf life (expiry date) of prepared volumetric solutions?

When I prepare a volumetric standard solution, I want to determine its expiry date by taking in consideration that it is not mentioned in any reference. And if possible I need a protocol for it.

03 April 2014 6,820 4 View

How to restore my column performance?

My column is Pico-Tag Column, 60Å, 4 µm, 3.9 mm X 150 mm used technique for HPLC amino acid analysis. Used in PicoTag® Amino Acid Analysis of Protein Hydrolysates.

31 December 2013 9,199 1 View

What is the official method for analysis of free amino acids by HPLC(WATERS)?

I need to know if this method is the official for analysis or not?

09 October 2013 6,915 5 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View