What can you do with PCR(Polymerase chain reaction) products?

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How Z-score is calculated for malnourished pediatric patients?

For a better understanding of z-score calculations for undernutrition.

31 December 2023 9,883 5 View

How I can increase sample size for a research by changing marginal errors?

To understand the relationship between change in marginal error and research sample size.

27 December 2023 8,678 3 View

How to be an effective peer reviewer for journals?

How to be an effective peer reviewer for journals? This question is aimed to understand: 1. Criteria to be an effective peer reviewer; 2. Skills needed to be an effective peer reviewer; and 3....

24 October 2023 6,625 6 View

I want to exfoliate bulk FeSe to monolayer. How I can do it?

I want to exfoliate bulk FeSe to monolayer. I tried different methods: Mechanical methods, Liquid Phase, but they are not working well. Please can you share an experience?

25 July 2023 9,989 3 View

How to convert UV-Vis DRS data to absorbance?

I have UV-Vis DRS data, but I am confused to convert this data to absorbance to plot Absorbance versus Wavelength. Is anyone who can help me, please?

09 May 2023 4,125 4 View

What is the percent error in the photodegradation efficiency?

Is it possible to calculate the percent error from the photodegradation efficiency during the photocatalytic degradation experiment?

28 March 2023 7,267 3 View

How to plot the Rietveld refinement of XRD data in Origin?

Scholars help me! I have done the Rietveld refinement by using GSAS-II software. How can I plot the refined data in Origin? I have tried many times but I couldn't do it.

27 March 2023 2,104 3 View

Is it possible to plot multiple rietveld refined XRD data by using GSAS-II?

I am using GSAS-II software for Rietveld refinement of my XRD data. I have refined different metal-doped ZnO. Is it possible to plot all refined data in the stacked form?

23 March 2023 5,694 0 View

What is the error involved in the estimation of crystallite size from XRD measurements?

I have calculated the crystallite size from my XRD data by using Debye Scherre's approximation. Is it necessary to calculate the percent error?

22 March 2023 7,958 5 View

Why are not statistically non-significant findings a candidate for publication?

Assume that one is building upon an already tested , statistically significant, peer reviewed and published idea (e.g. could be some X and Y relationships). Unfortunately, replication of the...

20 March 2022 1,264 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Identify the eight key processes of excellent supply chain management and discuss how each of these processes impacts the end customer?

Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26

04 August 2024 9,919 4 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Craig Silver

I don't think answering your homework questions is the best use of RG.

Laurence Stuart Dawkins-Hall

If this is a purely academic question then the following:

1. Sequence the product to confirm the identity of the DNA from which PCR fragment was derived

2. Clone into an expression vector if an open reading frame to express a fragment of protein for biochemical studies

There are many many (more) applications...

Katie A Burnette

So many possibilities!

But this sounds like an attempt to get answers to a homework question. Go read your textbook, look at product manuals for PCR kits, etc.