Does Location Map of Informal Settlements in South Africa exist?

I urgently need a map that shows the point locations of informal settlements in South Africa. I am currently working on a study that seeks to relate protests location to the location of informal...

02 February 2021 4,113 7 View

"The following block is not supported in Phasor simulation?

I am designing a control system for Hybrid renewable energy systems using Matlab/simulink/simscape, but currently having a problem. Whenever I run the simulation, I get the error shown below. "The...

12 January 2021 2,142 5 View

What methodology can be used in battling and managing security incidence in IoT technologies ?

I am writing a research paper on security incidence response and Management concerning IoT

24 December 2019 9,064 2 View

Can science provide answers to every questions? or Can science provide solution to every problems or issues of life?

There is that current and popular thinking and trend in research that every problem or issue of life that is not scientifically proved is not valid and correct. So, should every academic research...

10 October 2018 9,910 13 View

Method of dtermining cellulose in plant litter sample?

determination of cellulose in plant litter sample

08 November 2017 4,718 3 View

How do I construct a phylogenetic SNP tree using whole genome sequences of bacteria?

I have obtained assembled genomes (assembled contigs) of 14 salmonellae to construct a phylogenetic SNP tree but my background in this is little. I will appreciate some sorts of guides through the...

07 September 2015 9,527 15 View

ICP-AES operation standards

What type of material are sample vials and glasswares used in ICP-AES made of? What type of solution is used to adjust CID part in ICP-AES operation?

09 July 2012 3,726 7 View

I am in need of additionnal paper on the Mormyrids fish especially on their feeding ecology and their reproductive traits

01 January 1970 9,165 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View