I am working on bacteriophages. In my research, I am looking for a suitable lytic bacteriophage for Staphylococcus aureus. I have isolated a bacteriophage and it has formed a lot of clear plaque on the agar but when I want to enrich it or in the liquid phase, it is too turbid and it is not different from my control. Therefore, the MOI of my bacteriophage is a challenge. I don't know what to do!
It may be that you are not using the MOI, but it might also be that you are growing the cultures too long. Sometimes you can only see the visible lysis early on and then resistant mutants take over and grow to high density.
I would monitor the culture (after adding the phage) and check it at least every hour for the 6-8 hours to see whether you see lysis debris or not.
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Physical Phenomena
- Luminescence
- Fluorescence