Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Hye All, Greetings, How can i get surface run off water data?

this is for my thesis project, so this is the title of my thesis, the effect of land use on hydrological components in Muar, Johor, Malaysia any ideas, guidance, and suggestions all? Thank You, :)

02 February 2021 265 5 View

What is the difference of FTIR for functional properties and structural properties of protein?

I need to send my sample to outsource laboratory and how can i tell them that i want to run the FTIR for structural properties of protein? is there any differenece between FTIR for functional...

15 January 2021 8,992 1 View

What's the correct way of treating ordinal variable as continuous in regression?

08 January 2021 920 16 View

Does low pHPZC (Zero point charge) indicates higher negative charged surface ?

I found that, there is a difference in pHPZC value when higher impregnation ratio of acid on adsorbent surface. Lower pHPZC value when higher ratio of acid impregnation and adsorption performance...

05 January 2021 7,224 3 View

Why do I see many vacuoles on my cancer cells?

I am currently growing BICR31 cancer cells for conditioned media collection, and as I passaged it I have seen many bubbles/vacuoles - like structures. Cells were healthy during the first split...

05 January 2021 2,320 5 View

How can be the Virus COVID-19 become mutation?

The current situation the virus become mutation, but there were information that zero percent case in Wuhan?

31 December 2020 2,457 4 View

Why does activation process called as activation? what does the term means actually?

I'm referring to activated carbon activation. Activation process does increase the surface area and functional groups content in activated carbon compared to biochar and raw biomass. But I'm...

30 December 2020 6,514 1 View

I am looking for a standard ?

Dear Researchers, I am a researcher and I am working on a Xenon accelerated aging of a polymer and I need the ASTM G 155 standard. I will be grateful if someone can share it with me. Best...

28 December 2020 549 2 View

Explain the strain determined using directly the tensile machine test or the video extensometer ?

Dear Researchers, I would like to know the logical margin between the strain of a polymer in the tensile test determined through the traverse (the value from the machine itself) or using a video...

12 December 2020 3,636 4 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View