What can be used as binding buffer and washing buffer for DNA Aptamer in cell SELEX?

14 November 2018 1 3K Report

Cell line is SKOV3

usually, the binding buffer is the washing buffer adding 5mM MgCl2 and 0.1-0.5% Tween 80, the washing buffer is PBS adding 25mM Glucose, you can find the information from:

http://www.pnas.org/content/103/32/11838

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