I am trying to induce callus from woody plants grown in an arboretum. We have tried sterilizing explants using bleach but we are unable to get rid of funal contaminations.
The explants are woody stems. Are there better sterilization stategies we can adopt? Thanks for your help.
Neha Shukla
I have never used stems as explant but from previous lab experience on surface sterilization of seeds, mercury chloride treatment could help reduce/contain fungal and bacterial contaminants.
Or you can look up in these articles; the first one is a review:
Article Surface sterilization for isolation of endophytes: Ensuring ...
Article Efficient In Vitro Sterilization and Propagation from Stem S...
All the best.
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